Rat intestinal trehalase. Studies of the active site
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چکیده
منابع مشابه
Mechanism-Based Studies of the Active Site-Directed Inhibition and Activation of Enzyme Transketolase
Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...
متن کاملRat trehalase: cDNA cloning and mRNA expression in adult rat tissues and during intestinal ontogeny.
A partial rat trehalase cDNA has been cloned and used to examine trehalase mRNA expression. Northern blotting with total RNA from 11 adult rat tissues showed a trehalase transcript only in small intestine, where it was abundant in proximal regions but declined steeply toward the ileum. During development, trehalase mRNA was not detectable in jejunum until postnatal day 19 and then increased mar...
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چکیده ندارد.
15 صفحه اولStudies on the Active Site of Clostripain
Clostripain is rapidly and selectively inactivated by the chloromethyl ketone derived from cr-N-tosyl-L-lysine (TLCK) with an apparent second order rate constant of 8.7 X lo4 M-I set-i at pH 6.5. Alkylation by TLCK occurs only with catalytically active enzyme, the rate of which is considerably retarded by the competitive inhibitors, oc-Ntosyl-L-homoarginine methyl ester and benzamidine. In cont...
متن کاملStudies on the Active Site of Chymotrypsin.
as it rises in b to enter c above the midline, etc. It can be easily seen that a similar oscillation about the midline takes place in the rotating fluid annulus of Figure 4a and in the more general situation of Figure 4b. These oscillations can be made quite small by adjusting the density of the buffer to match the particle density and by speeding up the rate of buffer flow to shorten the perio...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2470715